Leptospira interrogans is a Gram-negative bacterie from phylum Spirochaetae, it is cause of leptospirosis (also known as field fever, rat catcher's yellows, and pretibial fever).
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Real time PCR kit for molecular diagnostics is a set of chemicals neccessary for detection of target RNA or DNA sequence. It is typically composed of set of PCR primers, hybridization probe and MasterMix.